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KMID : 0603820150210040188
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Journal of Experimental & Biomedical Science
2015 Volume.21 No. 4 p.188 ~ p.197
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Water Extract from Rice Bran Fermented with Lactobacillus plantarum Hong Inhibits Thromboxane A₂ Production Associated Microsomal Enzyme Activity in Human Platelets
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Kim Hyun-Hong
Hong Jeong-Hwa
Pajaree Ingkasupart
Lee Dong-Ha
Yeo Da-Na
Park Hwa-Jin
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Abstract
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In this study, we investigated the effect of rice bran water extract fermented with Lactobacillus plantarum Hong (RBLw), on activities of cyclooxygenase-1 (COX-1) and thromboxane A₂ synthase (TXAS), thromboxane A₂ (TXA₂) production associated microsomal enzymes and evaluated its the antiplatelet effect. RBLw, containing 13.5 mg of ferulic acid, dose-dependently inhibited ADP-induced platelet aggregation, and inhibited the production of TXA₂, an aggregation molecule. In addition, RBLw directly inhibited COX-1 activity in a dose-dependent manner, but not TXAS activity in platelet microsomal fraction having cytochrome c reductase (an endoplasmic reticulum marker enzyme) activity and expressing COX-1 (72 kDa) and TXAS (60.5 kDa) proteins. These results suggest that RBLw selectively inhibited the activity of COX-1 rather than TXAS to attenuate TXA₂ production in ADP-activated platelets. Thus, we demonstrate that RBLw might have direct COX-1 antagonistic function for platelet aggregation-mediated diseases, such as thrombosis, myocardial infarction, atherosclerosis, and ischemic cerebrovascular disease.
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KEYWORD
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Rice bran fermentation, Thromboxane A₂, Microsomal fraction, Cyclooxygenase-1, Thromboxane A₂ synthase
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